Statements (30)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
E. coli strain
|
| gptkbp:antibioticResistance |
gptkb:tetracycline
|
| gptkbp:availableOn |
chemically competent cells
electrocompetent cells |
| gptkbp:blueWhiteScreening |
yes
|
| gptkbp:catalogueNumber |
C3040H
C3040I |
| gptkbp:endA |
1
|
| gptkbp:endA1Mutation |
improves plasmid quality
|
| gptkbp:gene |
F′ proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR) recA1 endA1 gyrA96 thi-1 hsdR17
|
| gptkbp:gyrA |
96
|
| gptkbp:hsdR |
17
|
| https://www.w3.org/2000/01/rdf-schema#label |
NEB Stable
|
| gptkbp:lacIq |
present
|
| gptkbp:lacZΔM15 |
present
|
| gptkbp:mainVendors |
gptkb:New_England_Biolabs
|
| gptkbp:organism |
gptkb:Bacteria
|
| gptkbp:proA+B+ |
present
|
| gptkbp:recA |
1
|
| gptkbp:recA1Mutation |
reduces recombination
|
| gptkbp:suitableFor |
blue/white screening
propagation of lentiviral plasmids propagation of unstable inserts |
| gptkbp:thi-1 |
present
|
| gptkbp:Tn10 |
present
|
| gptkbp:Tn10Resistance |
gptkb:tetracycline
|
| gptkbp:transformationEfficiency |
high
|
| gptkbp:usedFor |
cloning unstable DNA
|
| gptkbp:bfsParent |
gptkb:Escherichia_coli_K-12
|
| gptkbp:bfsLayer |
8
|