Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biochemical assay
|
| gptkbp:alsoKnownAs |
gptkb:Lowry_protein_assay
|
| gptkbp:application |
biochemistry
molecular biology clinical laboratories |
| gptkbp:basedOn |
reduction of Folin–Ciocalteu reagent by tyrosine and tryptophan residues
reaction of protein with copper under alkaline conditions |
| gptkbp:contrastsWith |
gptkb:Bradford_assay
Bicinchoninic acid assay (BCA assay) |
| gptkbp:detects |
absorbance measurement at 750 nm
|
| gptkbp:developedBy |
gptkb:Oliver_H._Lowry
1951 |
| https://www.w3.org/2000/01/rdf-schema#label |
Lowry Process
|
| gptkbp:limitation |
sensitive to interfering substances such as detergents and buffers
|
| gptkbp:measures |
total protein concentration in solution
|
| gptkbp:principle |
colorimetric assay
|
| gptkbp:requires |
gptkb:Folin–Ciocalteu_reagent
alkaline copper tartrate solution |
| gptkbp:sensitivity |
5–100 micrograms of protein
|
| gptkbp:usedFor |
protein quantification
|
| gptkbp:bfsParent |
gptkb:John_D._Lowry
gptkb:Lowry_Digital |
| gptkbp:bfsLayer |
7
|