Statements (37)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Biophysical Technique
|
| gptkbp:advantage |
High Throughput Capability
Homogeneous Assay Format No Separation Step Required |
| gptkbp:affects |
Temperature
Viscosity Fluorophore Lifetime Molecular Weight |
| gptkbp:alternativeName |
FP Assay
Fluorescence Anisotropy Assay |
| gptkbp:basedOn |
Emission of Polarized Light
|
| gptkbp:detects |
Enzyme Activity
Ligand Binding Protein-Protein Interactions |
| gptkbp:discoveredIn |
1950s
|
| https://www.w3.org/2000/01/rdf-schema#label |
Fluorescence Polarization
|
| gptkbp:measures |
Binding Affinity
Molecular Interactions Molecular Size |
| gptkbp:quantifiedBy |
Anisotropy Value (r)
Polarization Value (P) |
| gptkbp:relatedTo |
Fluorescence Anisotropy
Fluorescence Resonance Energy Transfer |
| gptkbp:requires |
Fluorometer
Fluorescent Probe Polarization Filter Polarized Light Source |
| gptkbp:usedFor |
DNA-Protein Binding Assays
Enzyme Kinetics Measuring Antibody-Antigen Interactions Receptor-Ligand Binding Assays Screening Small Molecule Inhibitors |
| gptkbp:usedIn |
gptkb:Molecular_Biology
Biochemistry Drug Discovery |
| gptkbp:bfsParent |
gptkb:Surface_Plasmon_Resonance
|
| gptkbp:bfsLayer |
7
|