Statements (53)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
physical phenomenon
analytical technique |
| gptkbp:advantage |
real-time data
no labeling required |
| gptkbp:alternative_technique |
gptkb:Fluorescence_Polarization
gptkb:Isothermal_Titration_Calorimetry gptkb:Quartz_Crystal_Microbalance |
| gptkbp:analyte_type |
lipids
nucleic acids proteins small molecules |
| gptkbp:commercialized_by |
gptkb:Biacore
|
| gptkbp:common_metal |
gptkb:gold
silver |
| gptkbp:detects |
measures changes in refractive index
|
| gptkbp:field |
analytical chemistry
biochemistry physics |
| https://www.w3.org/2000/01/rdf-schema#label |
Surface Plasmon Resonance
|
| gptkbp:inventedBy |
1980s
|
| gptkbp:label-free |
true
|
| gptkbp:limitation |
surface sensitivity
non-specific binding requires purified samples |
| gptkbp:measures |
concentration
association rate binding affinity dissociation rate |
| gptkbp:output |
sensorgram
|
| gptkbp:principle |
excitation of surface plasmons by light
|
| gptkbp:real-time_monitoring |
true
|
| gptkbp:requires |
gptkb:Kretschmann_configuration
microfluidics optical sensor polarized light metallic surface optical detection system prism coupling |
| gptkbp:sensitivity |
high
|
| gptkbp:sensorgram_axis |
response units vs time
|
| gptkbp:surface_functionalization |
self-assembled monolayers
antibodies DNA probes |
| gptkbp:used_in |
food safety
material science environmental monitoring clinical diagnostics |
| gptkbp:usedFor |
biosensing
drug discovery molecular interaction analysis protein-ligand binding studies |
| gptkbp:bfsParent |
gptkb:SPR
|
| gptkbp:bfsLayer |
6
|