Statements (32)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Escherichia coli strain
|
| gptkbp:deficientIn |
endA1 (endonuclease I)
gyrA96 (DNA gyrase) hsdR17 (restriction system) lacZYA-argF (lac operon) phoA (alkaline phosphatase) recA1 (recombination) relA1 (stringent response) supE44 (amber suppressor tRNA) thi-1 (thiamine synthesis) λ- (lambda phage) |
| gptkbp:feature |
endA1 mutation for cleaner DNA preps
high transformation efficiency improved plasmid yield recA1 mutation for plasmid stability reduced recombination |
| gptkbp:gene |
F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 λ- thi-1 gyrA96 relA1
|
| gptkbp:hostedBy |
gptkb:blue-white_screening
plasmid DNA propagation |
| https://www.w3.org/2000/01/rdf-schema#label |
DH5α
|
| gptkbp:mainVendors |
gptkb:New_England_Biolabs
gptkb:Thermo_Fisher_Scientific gptkb:Promega gptkb:Takara_Bio |
| gptkbp:organism |
gptkb:Bacteria
|
| gptkbp:resistantTo |
ampicillin (if transformed with plasmid)
kanamycin (if transformed with plasmid) |
| gptkbp:taxonRank |
562 (E. coli)
|
| gptkbp:usedFor |
molecular cloning
|
| gptkbp:bfsParent |
gptkb:Escherichia_coli_K-12
gptkb:Escherichia_coli_(strain_K12) |
| gptkbp:bfsLayer |
8
|