Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:protein
gptkb:CRISPR-Cas_system |
| gptkbp:affiliatedWith |
gptkb:Type_V_CRISPR_system
Class 2 CRISPR system |
| gptkbp:alternativeName |
gptkb:Cpf1
CRISPR from Prevotella and Francisella 1 |
| gptkbp:cleavage |
double-stranded DNA
staggered cut |
| gptkbp:compatibleWith |
gptkb:tracrRNA
|
| gptkbp:discoveredBy |
gptkb:Emmanuelle_Charpentier
gptkb:Feng_Zhang |
| gptkbp:firstDescribed |
2015
|
| gptkbp:function |
genome editing
DNA cleavage |
| gptkbp:hasCollateralActivity |
single-stranded DNA cleavage
|
| https://www.w3.org/2000/01/rdf-schema#label |
Cas12a (Cpf1)
|
| gptkbp:orthologIn |
gptkb:Francisella_novicida
gptkb:Acidaminococcus_sp._BV3L6 gptkb:Lachnospiraceae_bacterium_ND2006 |
| gptkbp:produces |
sticky ends
|
| gptkbp:recognizesPAM |
TTTV (where V is A, C, or G)
|
| gptkbp:requires |
gptkb:crRNA
|
| gptkbp:usedIn |
gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform biosensing diagnostics genome engineering therapeutic genome editing base editing prime editing functional genomics point-of-care diagnostics epigenome editing gene knock-in gene knockout genetic screening nucleic acid detection mutation detection plant genome editing SARS-CoV-2 detection pathogen detection transcriptional regulation antiviral defense multiplex genome editing bacterial genome editing mammalian genome editing COVID-19 detection |
| gptkbp:bfsParent |
gptkb:type_V_CRISPR-Cas_system
|
| gptkbp:bfsLayer |
8
|