Statements (31)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:enzyme
|
| gptkbp:alternativeTo |
gptkb:NAD+-dependent_DNA_ligase
|
| gptkbp:catalyzes |
formation of phosphodiester bonds in DNA
|
| gptkbp:cofactor |
gptkb:ATP
|
| gptkbp:compatibleWith |
most archaea
|
| gptkbp:EC_number |
6.5.1.1
|
| gptkbp:foundIn |
gptkb:Bacteria
eukaryotes |
| gptkbp:function |
DNA repair
DNA replication |
| gptkbp:gene |
ligA (in E. coli)
|
| gptkbp:GO_term |
GO:0003911
|
| https://www.w3.org/2000/01/rdf-schema#label |
ATP-dependent DNA ligase
|
| gptkbp:inhibitedBy |
gptkb:EDTA
|
| gptkbp:mechanismOfAction |
forms DNA-adenylate intermediate
forms phosphodiester bond transfers AMP to DNA 5' phosphate |
| gptkbp:product |
gptkb:AMP
pyrophosphate ligated DNA |
| gptkbp:requires |
Mg2+
|
| gptkbp:structure |
multi-domain protein
|
| gptkbp:substrate |
gptkb:ATP
DNA with single-strand breaks |
| gptkbp:UniProtID |
P06836
|
| gptkbp:used_in |
molecular biology
cloning genetic engineering DNA fragment ligation |
| gptkbp:bfsParent |
gptkb:DNA_ligase
|
| gptkbp:bfsLayer |
7
|