lambda Red recombination system

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf genetic engineering tool
recombination system
gptkbp:Beta_protein_function single-strand annealing
gptkbp:composedOf gptkb:Beta_protein
gptkb:Exo_protein
gptkb:Gam_protein
gptkbp:discoveredBy gptkb:Donald_Court
gptkbp:discoveredIn 1998
gptkbp:enables homologous recombination
gptkbp:Exo_protein_function 5' to 3' exonuclease
gptkbp:foundIn gptkb:Bacteria
gptkbp:Gam_protein_function inhibits RecBCD
https://www.w3.org/2000/01/rdf-schema#label lambda Red recombination system
gptkbp:inhibitedBy host RecBCD nuclease
gptkbp:originatedIn gptkb:bacteriophage_lambda
gptkbp:requires short homology arms
gptkbp:usedFor genome engineering
gene knockout
gene replacement
recombineering
gptkbp:usedIn bacterial genetics
gptkbp:bfsParent gptkb:lambda_phage
gptkbp:bfsLayer 7