Statements (33)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing technology
|
| gptkbp:application |
gptkb:agricultural_machinery
gene therapy functional genomics |
| gptkbp:contrastsWith |
CRISPR genome editing
TALENs genome editing |
| gptkbp:deliveryMethods |
gptkb:gene
plasmid DNA viral vectors |
| gptkbp:developedBy |
gptkb:Sangamo_BioSciences
|
| gptkbp:enables |
gene knockout
gene correction gene insertion targeted DNA double-strand breaks |
| gptkbp:firstDescribed |
1996
|
| gptkbp:fullName |
Zinc Finger Nucleases genome editing
|
| https://www.w3.org/2000/01/rdf-schema#label |
ZFNs genome editing
|
| gptkbp:limitation |
off-target effects
complex protein design lower efficiency than CRISPR |
| gptkbp:mechanismOfAction |
DNA binding by zinc finger domains
DNA cleavage by FokI nuclease |
| gptkbp:notableFor |
crop improvement
HIV gene therapy clinical trials |
| gptkbp:patent |
gptkb:Sangamo_BioSciences
|
| gptkbp:requires |
custom protein engineering
|
| gptkbp:usedIn |
animal genome editing
plant genome editing human cell genome editing |
| gptkbp:uses |
FokI nuclease domain
engineered zinc finger proteins |
| gptkbp:bfsParent |
gptkb:CRISPR-Cas12_genome_editing_technology
|
| gptkbp:bfsLayer |
5
|