Statements (29)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology tool
|
| gptkbp:advantage |
high efficiency
fast protocol simple workflow |
| gptkbp:application |
cloning PCR products
expression vector construction subcloning DNA fragments |
| gptkbp:component |
competent cells
control DNA linearized vector reaction buffer topoisomerase I enzyme |
| gptkbp:feature |
ligation-independent cloning
topoisomerase I-based cloning no need for restriction enzymes no need for DNA ligase rapid cloning |
| https://www.w3.org/2000/01/rdf-schema#label |
TOPO cloning kits
|
| gptkbp:manufacturer |
gptkb:Thermo_Fisher_Scientific
gptkb:Invitrogen |
| gptkbp:method |
TA cloning
blunt-end cloning |
| gptkbp:relatedTo |
gptkb:Gateway_cloning
PCR cloning TA cloning kits |
| gptkbp:usedFor |
DNA cloning
|
| gptkbp:usedIn |
molecular biology
|
| gptkbp:bfsParent |
gptkb:Invitrogen
|
| gptkbp:bfsLayer |
7
|