Statements (33)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
high efficiency
no need for ligase no need for restriction enzymes |
| gptkbp:alternativeTo |
TOPO cloning
restriction enzyme cloning |
| gptkbp:basedOn |
site-specific recombination
|
| gptkbp:commercialUse |
gptkb:Thermo_Fisher_Scientific
|
| gptkbp:developedBy |
gptkb:Life_Technologies
|
| gptkbp:enables |
high-throughput cloning
transfer of DNA fragments between vectors |
| gptkbp:hasBiologicalActivity |
gptkb:lambda_integrase
gptkb:Cre_recombinase excisionase |
| https://www.w3.org/2000/01/rdf-schema#label |
Gateway cloning
|
| gptkbp:introducedIn |
late 1990s
|
| gptkbp:step |
BP reaction
LR reaction |
| gptkbp:usedFor |
DNA cloning
|
| gptkbp:usedIn |
synthetic biology
functional genomics protein expression plant transformation |
| gptkbp:uses |
attB site
attL site attP site attR site destination vector entry vector |
| gptkbp:bfsParent |
gptkb:attB
gptkb:TOPO gptkb:TOPO_cloning_kits |
| gptkbp:bfsLayer |
8
|