Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:enzyme
|
| gptkbp:activity |
removes nucleotides from 5′-termini
|
| gptkbp:alternativeName |
T7 gene 6 protein
gene 6 exonuclease |
| gptkbp:catalyzes |
exonucleolytic cleavage in the 5′- to 3′-direction
|
| gptkbp:cofactor |
Mg2+
|
| gptkbp:does_not_act_on |
single-stranded DNA
|
| gptkbp:EC_number |
3.1.11.6
|
| gptkbp:gene |
gene 6 of T7 phage
|
| https://www.w3.org/2000/01/rdf-schema#label |
T7 exonuclease
|
| gptkbp:inhibitedBy |
gptkb:EDTA
|
| gptkbp:molecularWeight |
approximately 60 kDa
|
| gptkbp:organism |
Enterobacteria phage T7
|
| gptkbp:removes |
5′-phosphorylated nucleotides
|
| gptkbp:structure |
homodimer
|
| gptkbp:substrate |
double-stranded DNA
|
| gptkbp:used_in |
molecular biology
cloning DNA sequencing DNA manipulation |
| gptkbp:usedFor |
generation of single-stranded overhangs
|
| gptkbp:bfsParent |
gptkb:DNA_exonuclease
|
| gptkbp:bfsLayer |
7
|