Statements (21)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA quantification method
|
| gptkbp:advantage |
cost-effective
simple protocol |
| gptkbp:alternativeTo |
TaqMan chemistry
|
| gptkbp:detects |
double-stranded DNA
|
| gptkbp:developedBy |
real-time monitoring of DNA amplification
|
| gptkbp:emissionWavelength |
~520 nm
|
| gptkbp:excitationWavelength |
~497 nm
|
| gptkbp:fluorescence |
upon binding to DNA
|
| https://www.w3.org/2000/01/rdf-schema#label |
SYBR Green chemistry
|
| gptkbp:limitation |
non-specific binding
cannot distinguish between specific and non-specific products |
| gptkbp:monitors |
PCR amplification
|
| gptkbp:requires |
melt curve analysis for specificity
|
| gptkbp:usedBy |
molecular biologists
|
| gptkbp:usedFor |
gene expression analysis
quantitative PCR (qPCR) |
| gptkbp:usedIn |
gptkb:real-time_PCR
|
| gptkbp:uses |
SYBR Green dye
|
| gptkbp:bfsParent |
gptkb:LightCycler
|
| gptkbp:bfsLayer |
7
|