Statements (17)
| Predicate | Object | 
|---|---|
| gptkbp:instanceOf | 
                                    
                                        
                                            gptkb:molecular_biology_technique
                                        
                                         | 
                            
| gptkbp:advantage | 
                                    
                                        
                                            
                                            cost-effective
                                        
                                        
                                         simple protocol  | 
                            
| gptkbp:alternativeTo | 
                                    
                                        
                                            gptkb:TaqMan_assays
                                        
                                         | 
                            
| gptkbp:detects | 
                                    
                                        
                                            
                                            fluorescence
                                        
                                        
                                         double-stranded DNA  | 
                            
| gptkbp:limitation | 
                                    
                                        
                                            
                                            non-specific binding
                                        
                                        
                                         cannot distinguish between specific and non-specific products  | 
                            
| gptkbp:requires | 
                                    
                                        
                                            
                                            melt curve analysis
                                        
                                        
                                         | 
                            
| gptkbp:usedFor | 
                                    
                                        
                                            gptkb:quantitative_PCR
                                        
                                         | 
                            
| gptkbp:usedIn | 
                                    
                                        
                                            
                                            gene expression analysis
                                        
                                        
                                         genotyping pathogen detection  | 
                            
| gptkbp:uses | 
                                    
                                        
                                            
                                            SYBR Green dye
                                        
                                        
                                         | 
                            
| gptkbp:bfsParent | 
                                    
                                        
                                            gptkb:7500_Real-Time_PCR_System
                                        
                                         | 
                            
| gptkbp:bfsLayer | 
                                    
                                        
                                            
                                            8
                                        
                                        
                                         | 
                            
| https://www.w3.org/2000/01/rdf-schema#label | 
                                    
                                        
                                            
                                            SYBR Green assays
                                        
                                        
                                         |