Statements (45)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
super-resolution microscopy technique
|
| gptkbp:abbreviation |
gptkb:STORM
|
| gptkbp:advantage |
quantitative analysis
high spatial resolution multicolor imaging |
| gptkbp:application |
neuroscience
structural biology cellular imaging protein localization |
| gptkbp:category |
fluorescence microscopy
super-resolution imaging |
| gptkbp:developedBy |
gptkb:Xiaowei_Zhuang
|
| gptkbp:enables |
nanoscale imaging
|
| gptkbp:field |
biophysics
cell biology microscopy |
| gptkbp:fullName |
gptkb:Stochastic_Optical_Reconstruction_Microscopy
|
| gptkbp:hasPatent |
gptkb:US_Patent_7,828,695
|
| https://www.w3.org/2000/01/rdf-schema#label |
STORM microscopy
|
| gptkbp:improves |
diffraction-limited fluorescence microscopy
|
| gptkbp:influencedBy |
development of new super-resolution techniques
|
| gptkbp:limitation |
photobleaching
requires specific fluorophores slow acquisition speed |
| gptkbp:notablePublication |
gptkb:Betzig_et_al.,_Science,_2006
gptkb:Rust,_Bates,_Zhuang,_Nature_Methods,_2006 |
| gptkbp:publishedIn |
2006
|
| gptkbp:relatedTo |
gptkb:PALM_microscopy
gptkb:GSDIM gptkb:SIM_microscopy gptkb:dSTORM single-molecule localization microscopy |
| gptkbp:requires |
high-precision localization algorithms
specialized imaging buffers |
| gptkbp:resolution |
~20-30 nanometers
|
| gptkbp:signature |
fixed cells
live cells |
| gptkbp:usedIn |
membrane protein organization
super-resolution imaging of microtubules synaptic structure studies |
| gptkbp:uses |
computational reconstruction
photoswitchable fluorophores sequential activation and imaging |
| gptkbp:bfsParent |
gptkb:STED_microscopy
|
| gptkbp:bfsLayer |
5
|