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gptkbp:instanceOf
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super-resolution microscopy technique
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gptkbp:application
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neuroscience research
protein localization
imaging of living cells
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gptkbp:category
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super-resolution imaging
advanced microscopy
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gptkbp:commercialUse
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gptkb:Abberior_Instruments
gptkb:Leica_Microsystems
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gptkbp:enables
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imaging of structures smaller than 50 nm
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gptkbp:field
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biophysics
cell biology
optical microscopy
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gptkbp:fullName
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gptkb:Stimulated_Emission_Depletion_microscopy
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https://www.w3.org/2000/01/rdf-schema#label
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STED microscopy
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gptkbp:improves
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gptkb:Abbe_diffraction_limit
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gptkbp:introducedIn
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1994
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gptkbp:inventedBy
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gptkb:Stefan_Hell
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gptkbp:NobelPrizeYear
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gptkb:2014_Nobel_Prize_in_Chemistry
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gptkbp:notablePublication
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Hell, S.W. & Wichmann, J. (1994). Optics Letters 19, 780-782.
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gptkbp:principle
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uses stimulated emission to deplete fluorescence in a controlled manner
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gptkbp:relatedTo
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gptkb:STORM_microscopy
gptkb:confocal_microscopy
gptkb:PALM_microscopy
fluorescence microscopy
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gptkbp:resolution
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below diffraction limit
nanoscale
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gptkbp:uses
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fluorescent dyes
laser beams
depletion laser
excitation laser
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gptkbp:bfsParent
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gptkb:Stefan_Hell
gptkb:Stefan_W._Hell
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gptkbp:bfsLayer
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4
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