Polymerase chain reaction (PCR)

URI: https://gptkb.org/entity/Polymerase_chain_reaction_%28PCR%29
GPTKB entity
Statements (45)
Predicate Object
gptkbp:instance_of gptkb:Biology
gptkbp:application gptkb:forensic_science
gptkb:research
genetic testing
medical diagnostics
cloning
gene expression analysis
pathogen detection
gptkbp:average_temperature typically 50-65° C for annealing
typically 72° C for extension
typically 94-98° C for denaturation
gptkbp:developed_by gptkb:Kary_Mullis
gptkbp:first_introduced gptkb:1983
gptkbp:has_limitations contamination risk
requires specific primers
not suitable for all DNA types
https://www.w3.org/2000/01/rdf-schema#label Polymerase chain reaction (PCR)
gptkbp:improves real-time PCR
fast PCR techniques
high-fidelity polymerases
isothermal amplification
gptkbp:involves thermal cycling
gptkbp:products PCR products
DNA copies
amplified DNA fragments
gptkbp:protocol gptkb:cooperative
annealing
denaturation
gptkbp:related_to gptkb:gene_therapy
gptkb:biotechnology
DNA sequencing
molecular diagnostics
genomics
gptkbp:requires gptkb:RNA_Polymerase
nucleotides
DNA template
primers
gptkbp:type_of multiplex PCR
digital PCR
nested PCR
quantitative PCR (q PCR)
reverse transcription PCR (RT-PCR)
gptkbp:used_for amplifying DNA
gptkbp:bfsParent gptkb:Kary_B._Mullis
gptkbp:bfsLayer 5