Pacific Biosciences SMRT sequencing
GPTKB entity
Statements (39)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencer
|
| gptkbp:abbreviation |
gptkb:SMRT_sequencing
|
| gptkbp:averageReadLength |
10,000-25,000 base pairs
|
| gptkbp:competitor |
Illumina sequencing
Oxford Nanopore sequencing |
| gptkbp:detects |
DNA methylation
fluorescently labeled nucleotides |
| gptkbp:developedBy |
gptkb:Pacific_Biosciences
|
| gptkbp:errorDetection |
random errors
~10-15% (raw) |
| gptkbp:fullName |
gptkb:Single_Molecule_Real-Time_sequencing
|
| https://www.w3.org/2000/01/rdf-schema#label |
Pacific Biosciences SMRT sequencing
|
| gptkbp:introducedIn |
2010
|
| gptkbp:libraryPreparation |
gptkb:SMRTbell_templates
|
| gptkbp:mainActivity |
de novo genome assembly
full-length transcript sequencing structural variant detection epigenetic modification detection |
| gptkbp:notableFeature |
real-time sequencing
single-molecule sequencing long read lengths no amplification required |
| gptkbp:platform |
gptkb:PacBio_RS
gptkb:PacBio_Sequel gptkb:PacBio_Sequel_II |
| gptkbp:readBy |
long reads
|
| gptkbp:readLength |
up to 100,000 base pairs
|
| gptkbp:sequencingMethod |
real-time detection of nucleotide incorporation
|
| gptkbp:sequencingType |
third-generation sequencing
|
| gptkbp:templateType |
circular DNA templates
|
| gptkbp:usedIn |
metagenomics
human genome sequencing plant genome sequencing microbial genome sequencing isoform sequencing (Iso-Seq) |
| gptkbp:uses |
zero-mode waveguides
|
| gptkbp:website |
https://www.pacb.com/smrt-science/smrt-sequencing/
|
| gptkbp:bfsParent |
gptkb:SMRTbell_templates
|
| gptkbp:bfsLayer |
7
|