Statements (33)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencer
|
| gptkbp:advantage |
direct detection of base modifications
long reads |
| gptkbp:application |
de novo genome assembly
haplotype phasing isoform sequencing |
| gptkbp:competitor |
Illumina sequencing
Oxford Nanopore sequencing |
| gptkbp:detects |
DNA methylation
base modifications |
| gptkbp:developedBy |
gptkb:Pacific_Biosciences
|
| gptkbp:enables |
long-read sequencing
real-time sequencing |
| gptkbp:errorDetection |
consensus sequencing
~10-15% (raw reads) |
| https://www.w3.org/2000/01/rdf-schema#label |
SMRT sequencing
|
| gptkbp:introducedIn |
2011
|
| gptkbp:libraryPreparation |
gptkb:SMRTbell_templates
|
| gptkbp:limitation |
higher cost per base
higher error rate (raw reads) |
| gptkbp:platform |
gptkb:PacBio_RS
gptkb:PacBio_Sequel gptkb:PacBio_Sequel_II |
| gptkbp:readLength |
up to 100,000 bases
|
| gptkbp:sequencingChemistry |
phospholinked nucleotides
|
| gptkbp:standsFor |
gptkb:Single_Molecule_Real-Time_sequencing
|
| gptkbp:usedFor |
genome assembly
epigenetic studies full-length transcript sequencing structural variant detection |
| gptkbp:uses |
zero-mode waveguides
|
| gptkbp:bfsParent |
gptkb:Pacific_Biosciences
|
| gptkbp:bfsLayer |
5
|