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gptkbp:instanceOf
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gptkb:DNA_sequencer
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gptkbp:advantage
|
direct detection of base modifications
long reads
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gptkbp:application
|
de novo genome assembly
haplotype phasing
isoform sequencing
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gptkbp:competitor
|
Illumina sequencing
Oxford Nanopore sequencing
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gptkbp:detects
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DNA methylation
base modifications
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gptkbp:developedBy
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gptkb:Pacific_Biosciences
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gptkbp:enables
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long-read sequencing
real-time sequencing
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gptkbp:errorDetection
|
consensus sequencing
~10-15% (raw reads)
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gptkbp:introducedIn
|
2011
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gptkbp:libraryPreparation
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gptkb:SMRTbell_templates
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gptkbp:limitation
|
higher cost per base
higher error rate (raw reads)
|
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gptkbp:platform
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gptkb:PacBio_RS
gptkb:PacBio_Sequel
gptkb:PacBio_Sequel_II
|
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gptkbp:readLength
|
up to 100,000 bases
|
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gptkbp:sequencingChemistry
|
phospholinked nucleotides
|
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gptkbp:standsFor
|
gptkb:Single_Molecule_Real-Time_sequencing
|
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gptkbp:usedFor
|
gptkb:genome_assembly
epigenetic studies
full-length transcript sequencing
structural variant detection
|
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gptkbp:uses
|
zero-mode waveguides
|
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gptkbp:bfsParent
|
gptkb:Pacific_Biosciences
|
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gptkbp:bfsLayer
|
5
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|
https://www.w3.org/2000/01/rdf-schema#label
|
SMRT sequencing
|