Statements (28)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
NMR experiment
|
| gptkbp:advantage |
high sensitivity
fast acquisition |
| gptkbp:application |
assignment of NMR resonances
structure determination of biomolecules |
| gptkbp:category |
gptkb:multidimensional_NMR
correlation spectroscopy |
| gptkbp:commonSample |
13C-labeled proteins
15N-labeled proteins |
| gptkbp:detects |
correlations between 1H and heteronuclei (usually 15N or 13C)
|
| gptkbp:dimensions |
2D NMR experiment
|
| gptkbp:fullName |
Heteronuclear Single Quantum Coherence
|
| https://www.w3.org/2000/01/rdf-schema#label |
HSQC
|
| gptkbp:introducedIn |
1980
|
| gptkbp:inventedBy |
Lloyd Kay
|
| gptkbp:limitation |
overlap in crowded spectra
requires isotopic labeling for biomolecules |
| gptkbp:relatedTo |
gptkb:NOESY
gptkb:TOCSY HMQC |
| gptkbp:spectrumAxes |
gptkb:1H
heteronucleus (15N or 13C) |
| gptkbp:usedIn |
protein NMR spectroscopy
|
| gptkbp:uses |
phase-sensitive detection
pulsed field gradients |
| gptkbp:bfsParent |
gptkb:multidimensional_NMR
gptkb:solution_NMR |
| gptkbp:bfsLayer |
7
|