Fluorescence in situ hybridization
GPTKB entity
Statements (52)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
gptkb:FISH
|
| gptkbp:alternativeTo |
gptkb:Southern_blot
PCR chromogenic in situ hybridization |
| gptkbp:appliesTo |
cells
chromosomes tissue sections |
| gptkbp:canBe |
formalin-fixed paraffin-embedded tissue
interphase nuclei fresh cells metaphase spreads |
| gptkbp:detects |
nucleic acids
aneuploidy chromosomal translocations gene amplifications gene deletions |
| gptkbp:developedBy |
1980s
|
| gptkbp:enables |
multiplexing with different fluorophores
simultaneous detection of multiple targets |
| gptkbp:firstDescribed |
1986
David Pinkel |
| https://www.w3.org/2000/01/rdf-schema#label |
Fluorescence in situ hybridization
|
| gptkbp:limitation |
requires specialized equipment
limited resolution compared to sequencing probe design complexity signal fading over time |
| gptkbp:recognizedBy |
chromosomal rearrangements
microbial species |
| gptkbp:relatedTo |
cancer diagnosis
prenatal diagnosis genetic diagnostics |
| gptkbp:requires |
washing steps
fluorescent labeling denaturation of DNA hybridization of probe |
| gptkbp:usedFor |
gene expression analysis
chromosome mapping detecting and localizing specific DNA sequences identifying chromosomal abnormalities |
| gptkbp:usedIn |
cytogenetics
microbiology oncology pathology plant genetics prenatal screening bacterial identification |
| gptkbp:uses |
fluorescent probes
|
| gptkbp:visualizes |
fluorescence microscopy
|
| gptkbp:bfsParent |
gptkb:Comparative_Genomic_Hybridization
gptkb:FISH_test |
| gptkbp:bfsLayer |
8
|