Statements (19)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biochemical assay
|
| gptkbp:absorbanceWavelength |
412 nm
|
| gptkbp:alsoKnownAs |
Ellman's reagent assay
|
| gptkbp:application |
protein quantification
enzyme activity assays glutathione measurement |
| gptkbp:citation |
Ellman, G.L. (1959) Arch. Biochem. Biophys. 82: 70–77
|
| gptkbp:colorimetric |
yes
|
| gptkbp:detects |
free sulfhydryl groups
|
| gptkbp:developedBy |
1959
George L. Ellman |
| gptkbp:fullNameOfReagent |
5,5'-dithiobis-(2-nitrobenzoic acid)
|
| https://www.w3.org/2000/01/rdf-schema#label |
Ellman method
|
| gptkbp:reactionProduct |
gptkb:TNB
|
| gptkbp:reactionProductColor |
yellow
|
| gptkbp:usedFor |
measuring thiol groups
|
| gptkbp:usesReagent |
DTNB
|
| gptkbp:bfsParent |
gptkb:plasma_cholinesterase
|
| gptkbp:bfsLayer |
8
|