|
gptkbp:instanceOf
|
gptkb:Bacteria
|
|
gptkbp:antibioticResistance
|
none (unless plasmid present)
|
|
gptkbp:contains
|
DE3 lysogen
|
|
gptkbp:DE3_lysogen
|
lambda prophage with T7 RNA polymerase gene
|
|
gptkbp:deficientIn
|
proteases Lon and OmpT
restriction system
|
|
gptkbp:expressionSystem
|
T7 RNA polymerase system
|
|
gptkbp:gene
|
F- ompT hsdSB (rB- mB-) gal dcm (DE3)
|
|
gptkbp:growthTemperature
|
37°C
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
E. coli BL21(DE3)
|
|
gptkbp:mainVendors
|
gptkb:Thermo_Fisher_Scientific
gptkb:NEB
Novagen
|
|
gptkbp:notableFeature
|
easy transformation
high-level protein expression
low protease activity
|
|
gptkbp:organism
|
gptkb:Bacteria
|
|
gptkbp:parentStrain
|
gptkb:E._coli_BL21
|
|
gptkbp:plasmidCompatibility
|
T7 promoter-based vectors
|
|
gptkbp:resistantTo
|
T1 phage
|
|
gptkbp:T7_RNA_polymerase
|
inducible by IPTG
|
|
gptkbp:usedFor
|
protein expression
recombinant protein production
|
|
gptkbp:usedIn
|
gptkb:biotechnology
molecular biology
synthetic biology
|
|
gptkbp:bfsParent
|
gptkb:Escherichia_coli_B
|
|
gptkbp:bfsLayer
|
8
|