Statements (26)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
protein assay
|
| gptkbp:absorbanceMaximum |
595 nm
|
| gptkbp:advantage |
gptkb:fire
simple sensitive |
| gptkbp:alternativeTo |
gptkb:Lowry_protein_assay
gptkb:Bicinchoninic_acid_assay |
| gptkbp:application |
biochemistry
molecular biology |
| gptkbp:basedOn |
Coomassie Brilliant Blue dye binding
|
| gptkbp:citation |
Analytical Biochemistry, 1976, 72(1-2):248-254
|
| gptkbp:detects |
colorimetric
|
| gptkbp:developedBy |
gptkb:Marion_M._Bradford
|
| gptkbp:dyeUsed |
gptkb:Coomassie_Brilliant_Blue_G-250
|
| https://www.w3.org/2000/01/rdf-schema#label |
Bradford protein assay
|
| gptkbp:introducedIn |
1976
|
| gptkbp:limitation |
interference by detergents
nonlinear response at high protein concentrations |
| gptkbp:measures |
protein concentration
|
| gptkbp:supportsProtocol |
incubate
measure absorbance at 595 nm mix sample with dye reagent |
| gptkbp:type |
biological samples
|
| gptkbp:usedFor |
protein quantification
|
| gptkbp:bfsParent |
gptkb:Denise_Clark_Bradford
|
| gptkbp:bfsLayer |
6
|