Statements (21)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular biology technique
|
| gptkbp:developedBy |
1980s
|
| gptkbp:enables |
cloning of full-length cDNA
determination of transcription start sites |
| gptkbp:fullName |
5' Rapid Amplification of cDNA Ends
|
| https://www.w3.org/2000/01/rdf-schema#label |
5' RACE
|
| gptkbp:publishedIn |
Frohman et al., 1988
|
| gptkbp:relatedTo |
gptkb:RT-PCR
3' RACE |
| gptkbp:requires |
reverse transcriptase
DNA polymerase RNA template gene-specific primer |
| gptkbp:step |
reverse transcription
PCR amplification cDNA synthesis |
| gptkbp:usedFor |
identifying 5' ends of RNA transcripts
|
| gptkbp:usedIn |
gene expression analysis
transcript mapping |
| gptkbp:bfsParent |
gptkb:Transcription_Start_Site
|
| gptkbp:bfsLayer |
7
|