Statements (25)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencing method
|
| gptkbp:alsoKnownAs |
chain-termination method
|
| gptkbp:application |
genome sequencing
genetic research mutation detection |
| gptkbp:detects |
gel electrophoresis
capillary electrophoresis |
| gptkbp:developedBy |
gptkb:Frederick_Sanger
|
| https://www.w3.org/2000/01/rdf-schema#label |
Sanger method
|
| gptkbp:introducedIn |
1977
|
| gptkbp:limitation |
expensive for large-scale sequencing
low throughput time-consuming for large genomes |
| gptkbp:NobelPrizeYear |
Frederick Sanger won Nobel Prize in Chemistry for DNA sequencing
|
| gptkbp:replacedBy |
next-generation sequencing
|
| gptkbp:sequencingReadLength |
up to 1000 base pairs
|
| gptkbp:sequencingType |
first-generation sequencing
|
| gptkbp:usedFor |
DNA sequencing
|
| gptkbp:uses |
DNA polymerase
primers dideoxynucleotides labeled nucleotides template DNA |
| gptkbp:bfsParent |
gptkb:Frederick_Sanger
|
| gptkbp:bfsLayer |
5
|