Reverse Phase Liquid Chromatography
GPTKB entity
Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Chromatography technique
|
| gptkbp:abbreviation |
gptkb:RPLC
|
| gptkbp:advantage |
Good resolution
High reproducibility Wide applicability |
| gptkbp:application |
Environmental analysis
Food analysis Peptide analysis Pharmaceutical quality control Protein separation |
| gptkbp:commonMobilePhaseSolvent |
gptkb:water
Methanol Acetonitrile |
| gptkbp:commonStationaryPhaseMaterial |
C18 silica
C8 silica |
| gptkbp:detects |
gptkb:UV-Vis_spectroscopy
Mass spectrometry |
| gptkbp:elutionOrder |
Non-polar compounds elute last
Polar compounds elute first |
| gptkbp:equipment |
UV detector
Autosampler Column oven HPLC system |
| https://www.w3.org/2000/01/rdf-schema#label |
Reverse Phase Liquid Chromatography
|
| gptkbp:inventedBy |
gptkb:Csaba_Horváth
1960s |
| gptkbp:limitation |
Not suitable for highly polar compounds
Limited for ionic compounds without ion-pairing agents |
| gptkbp:mainIngredient |
Formic acid
Phosphoric acid Trifluoroacetic acid |
| gptkbp:minimumPressure |
Up to 6000 psi
|
| gptkbp:mobilePhase |
gptkb:Polar
|
| gptkbp:regulates |
ICH guidelines
USP (United States Pharmacopeia) |
| gptkbp:relatedTo |
gptkb:High_Performance_Liquid_Chromatography
Normal Phase Liquid Chromatography |
| gptkbp:separates |
Molecules based on hydrophobicity
|
| gptkbp:stationaryPhase |
Non-polar
|
| gptkbp:temperature |
Room temperature to 60°C
|
| gptkbp:type |
Capillary column
Packed column |
| gptkbp:usedFor |
Qualitative analysis
Quantitative analysis |
| gptkbp:usedIn |
Biochemistry
Analytical chemistry Pharmaceutical analysis |
| gptkbp:bfsParent |
gptkb:RPLC
|
| gptkbp:bfsLayer |
7
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