RPKM

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf gene expression quantification method
gptkbp:alternativeTo TPM
FPKM
gptkbp:appliesTo single-end RNA-Seq data
gptkbp:C number of reads mapped to a gene
gptkbp:criticizedFor bias in multi-mapped reads
not suitable for comparing across samples
gptkbp:field genomics
bioinformatics
gptkbp:form RPKM = (10^9 * C) / (N * L)
https://www.w3.org/2000/01/rdf-schema#label RPKM
gptkbp:introduced Mortazavi et al.
gptkbp:introducedIn 2008
gptkbp:L length of the gene in base pairs
gptkbp:name total mapped reads in the experiment
gptkbp:normalizesFor sequencing depth
gene length
gptkbp:replacedBy TPM
gptkbp:standsFor Reads Per Kilobase of transcript, per Million mapped reads
gptkbp:usedFor normalizing gene expression levels
gptkbp:usedIn RNA-Seq
gptkbp:bfsParent gptkb:RK_Puram_Metro_Station
gptkbp:bfsLayer 7