Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
E. coli strain
|
| gptkbp:alsoKnownAs |
NEB 5-alpha Competent E. coli (High Efficiency)
|
| gptkbp:antibioticResistance |
none
|
| gptkbp:application |
cloning
plasmid propagation |
| gptkbp:feature |
endA1 mutation for improved plasmid quality
gyrA96 mutation for nalidixic acid resistance hsdR17 mutation for restriction-deficiency lacZΔM15 for blue/white screening not suitable for BAC or cosmid cloning not suitable for phage display not suitable for protein expression recA1 mutation for reduced recombination relA1 mutation for stringent response suitable for blue/white screening thi-1 mutation for thiamine requirement |
| gptkbp:gene |
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
|
| https://www.w3.org/2000/01/rdf-schema#label |
NEB C3020
|
| gptkbp:manufacturer |
gptkb:New_England_Biolabs
|
| gptkbp:storage |
-80°C
|
| gptkbp:transformationEfficiency |
>10^9 cfu/μg DNA
|
| gptkbp:bfsParent |
gptkb:Escherichia_coli_K-12
|
| gptkbp:bfsLayer |
8
|