NEB C3013

GPTKB entity

Statements (25)
Predicate Object
gptkbp:instanceOf E. coli strain
gptkbp:antibioticResistance none
gptkbp:application gptkb:transformation
cloning
gptkbp:catalogueNumber C3013
gptkbp:feature blue/white screening
endA1 mutation for improved plasmid quality
gyrA96 mutation for nalidixic acid resistance
high transformation efficiency
hsdR17 mutation for unmethylated DNA acceptance
lacZΔM15 for α-complementation
recA1 mutation for reduced recombination
supE44 mutation for amber suppression
thi-1 mutation for thiamine requirement
tonA mutation for T1 phage resistance
relA1 mutation for relaxed control of RNA synthesis
λ– (lambda negative)
gptkbp:fullName E. coli DH5α Competent Cells
gptkbp:gene F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 tonA
https://www.w3.org/2000/01/rdf-schema#label NEB C3013
gptkbp:manufacturer gptkb:New_England_Biolabs
gptkbp:storage -80°C
gptkbp:transformationEfficiency >10^8 cfu/μg DNA
gptkbp:bfsParent gptkb:Escherichia_coli_K-12
gptkbp:bfsLayer 8