MLPA (Multiplex Ligation-dependent Probe Amplification)
GPTKB entity
Statements (36)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
high throughput
cost effective requires small amount of DNA |
| gptkbp:alternativeTo |
gptkb:FISH
qPCR array CGH |
| gptkbp:analyzes |
up to 50 nucleic acid sequences in one reaction
|
| gptkbp:detects |
aneuploidy
exon deletions exon duplications methylation status |
| gptkbp:developedBy |
MRC Holland
|
| https://www.w3.org/2000/01/rdf-schema#label |
MLPA (Multiplex Ligation-dependent Probe Amplification)
|
| gptkbp:introducedIn |
2002
|
| gptkbp:limitation |
cannot detect balanced translocations
cannot detect point mutations limited to known sequences |
| gptkbp:relatedTo |
DNA methylation analysis
SNP genotyping gene dosage analysis |
| gptkbp:requires |
DNA sample
|
| gptkbp:usedFor |
detection of deletions
detection of duplications detection of copy number variations detection of methylation changes |
| gptkbp:usedIn |
cancer research
prenatal testing genetic diagnostics hereditary disease testing |
| gptkbp:uses |
capillary electrophoresis
PCR amplification ligation probe pairs |
| gptkbp:bfsParent |
gptkb:DMD_gene
|
| gptkbp:bfsLayer |
7
|