HiFi sequencing

GPTKB entity

Statements (30)
Predicate Object
gptkbp:instanceOf DNA sequencer
gptkbp:advantage low error rate
detection of complex variants
resolution of repetitive regions
gptkbp:alsoKnownAs High-Fidelity sequencing
gptkbp:application genome assembly
variant detection
phasing haplotypes
structural variant analysis
gptkbp:book SMRTbell libraries
gptkbp:contrastsWith Oxford Nanopore sequencing
Illumina short-read sequencing
gptkbp:developedBy gptkb:Pacific_Biosciences
gptkbp:feature high accuracy
long read lengths
gptkbp:firstReleased 2019
https://www.w3.org/2000/01/rdf-schema#label HiFi sequencing
gptkbp:output circular consensus sequencing (CCS) reads
gptkbp:platform gptkb:PacBio_Sequel_II
PacBio Sequel IIe
gptkbp:readAccuracy >99.9%
gptkbp:readLength 10-25 kb
gptkbp:technology gptkb:Single_Molecule,_Real-Time_(SMRT)_sequencing
gptkbp:type long-read, high-accuracy DNA sequences
gptkbp:usedIn human genome sequencing
plant genome sequencing
microbial genome sequencing
gptkbp:bfsParent gptkb:Pacific_Biosciences_of_California,_Inc.
gptkb:SMRTbell_templates
gptkbp:bfsLayer 7