ZN stain
E928060
ZN stain is a microbiological staining technique used to detect acid-fast organisms, particularly Mycobacterium species such as the tuberculosis bacillus.
All labels observed (2)
| Label | Occurrences |
|---|---|
| Neelsen component of Ziehl–Neelsen stain | 1 |
| ZN stain canonical | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T11466587 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: ZN stain Context triple: [Ziehl–Neelsen stain, alsoKnownAs, ZN stain]
-
A.
Stains
Stains is a suburban commune in the northern outskirts of Paris, France, known for its residential neighborhoods and diverse population.
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B.
Stain
Stain is a minor character from the animated television series "Rocket Power," known for being part of the show's ensemble of kids involved in extreme sports adventures.
-
C.
ZNA
ZNA is the IATA airport code for Nanaimo Harbour Water Airport, a seaplane facility serving Nanaimo, British Columbia, Canada.
-
D.
Tinte
Tinte is a small village in the Dutch province of South Holland, known for its rural character and annual local festivities.
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E.
AZO
AZO is the IATA airport code for Kalamazoo/Battle Creek International Airport in Michigan, United States.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: ZN stain Target entity description: ZN stain is a microbiological staining technique used to detect acid-fast organisms, particularly Mycobacterium species such as the tuberculosis bacillus.
-
A.
Stains
Stains is a suburban commune in the northern outskirts of Paris, France, known for its residential neighborhoods and diverse population.
-
B.
Stain
Stain is a minor character from the animated television series "Rocket Power," known for being part of the show's ensemble of kids involved in extreme sports adventures.
-
C.
ZNA
ZNA is the IATA airport code for Nanaimo Harbour Water Airport, a seaplane facility serving Nanaimo, British Columbia, Canada.
-
D.
Tinte
Tinte is a small village in the Dutch province of South Holland, known for its rural character and annual local festivities.
-
E.
AZO
AZO is the IATA airport code for Kalamazoo/Battle Creek International Airport in Michigan, United States.
- F. None of above. chosen
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
acid-fast staining method
ⓘ
differential staining method ⓘ microbiological staining technique ⓘ |
| advantage | high specificity for acid-fast bacilli ⓘ |
| alternativeName | acid-fast bacilli stain ⓘ |
| appliedTo |
clinical samples suspected of mycobacterial infection
ⓘ
sputum smears ⓘ tissue sections ⓘ |
| biosafetyConsideration | requires handling of potentially infectious aerosols ⓘ |
| category | hot acid-fast staining method ⓘ |
| colorOfBackground | blue or green depending on counterstain ⓘ |
| colorOfOrganisms | bright red rods ⓘ |
| comparedWith | Auramine–rhodamine fluorescent stain ⓘ |
| detects |
Mycobacterium leprae
ⓘ
Nocardia species (partially acid-fast) ⓘ
linked to:
Nocardia
other acid-fast bacteria ⓘ |
| fieldOfUse |
clinical microbiology
ⓘ
histopathology ⓘ infectious disease diagnostics ⓘ |
| fullName | Ziehl–Neelsen stain ⓘ |
| hasVariant |
Kinyoun stain
ⓘ
cold Ziehl–Neelsen method ⓘ
linked to:
Kinyoun stain
|
| limitation | lower sensitivity than some fluorescent staining methods ⓘ |
| microscopyType | light microscopy ⓘ |
| namedAfter |
Franz Ziehl
NERFINISHED
ⓘ
Friedrich Neelsen ⓘ |
| observationMagnification | oil immersion microscopy ⓘ |
| principle |
acid-fast organisms retain primary stain despite acid-alcohol decolorization
ⓘ
high mycolic acid content in cell wall confers acid-fastness ⓘ |
| samplePreparation | smear fixation by heat ⓘ |
| stainingTarget | mycolic acid–rich cell walls ⓘ |
| step |
application of carbol fuchsin with heating
ⓘ
counterstaining with methylene blue or malachite green ⓘ decolorization with acid-alcohol ⓘ |
| usedFor |
demonstration of acid-fast bacilli in clinical specimens
ⓘ
detection of Mycobacterium species ⓘ detection of Mycobacterium tuberculosis ⓘ detection of acid-fast organisms ⓘ diagnosis of tuberculosis ⓘ |
| usedIn | resource-limited settings for TB diagnosis ⓘ |
| usesCounterstain |
malachite green
ⓘ
methylene blue ⓘ |
| usesDecolorizer | acid-alcohol ⓘ |
| usesMordantOrAid | heat ⓘ |
| usesPrimaryStain | carbol fuchsin ⓘ |
| visualResult |
acid-fast bacilli appear red
ⓘ
non–acid-fast elements appear blue or green depending on counterstain ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: ZN stain Description of subject: ZN stain is a microbiological staining technique used to detect acid-fast organisms, particularly Mycobacterium species such as the tuberculosis bacillus.
Referenced by (2)
Full triples — surface form annotated when it differs from this entity's canonical label.
linked to: ZN stain