green fluorescent protein
E854252
Green fluorescent protein (GFP) is a naturally fluorescent protein originally found in jellyfish that is widely used as a marker in molecular and cell biology to visualize gene expression and protein localization.
All labels observed (2)
| Label | Occurrences |
|---|---|
| green fluorescent protein canonical | 2 |
| enhanced GFP | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T10305122 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: green fluorescent protein Context triple: [Roger Y. Tsien, knownFor, green fluorescent protein]
-
A.
Aequorea victoria
Aequorea victoria is a bioluminescent hydrozoan jellyfish best known as the natural source of green fluorescent protein (GFP), a crucial tool in molecular and cellular biology.
-
B.
Gi protein
Gi protein is a subtype of heterotrimeric G protein that inhibits adenylyl cyclase activity and modulates various intracellular signaling pathways when activated by G protein–coupled receptors.
-
C.
Flavin
Flavin is a surname most notably associated with American minimalist artist Dan Flavin, renowned for his fluorescent light installations.
-
D.
Fluorescent Adolescent
"Fluorescent Adolescent" is a popular indie rock song by Arctic Monkeys, known for its nostalgic lyrics about fading youth and its catchy, melodic guitar-driven sound.
-
E.
Yallow
Yallow is a surname associated with the individual known by the name or handle "w1n5t0n."
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: green fluorescent protein Target entity description: Green fluorescent protein (GFP) is a naturally fluorescent protein originally found in jellyfish that is widely used as a marker in molecular and cell biology to visualize gene expression and protein localization.
-
A.
Aequorea victoria
Aequorea victoria is a bioluminescent hydrozoan jellyfish best known as the natural source of green fluorescent protein (GFP), a crucial tool in molecular and cellular biology.
-
B.
Gi protein
Gi protein is a subtype of heterotrimeric G protein that inhibits adenylyl cyclase activity and modulates various intracellular signaling pathways when activated by G protein–coupled receptors.
-
C.
Flavin
Flavin is a surname most notably associated with American minimalist artist Dan Flavin, renowned for his fluorescent light installations.
-
D.
Fluorescent Adolescent
"Fluorescent Adolescent" is a popular indie rock song by Arctic Monkeys, known for its nostalgic lyrics about fading youth and its catchy, melodic guitar-driven sound.
-
E.
Yallow
Yallow is a surname associated with the individual known by the name or handle "w1n5t0n."
- F. None of above. chosen
Statements (52)
| Predicate | Object |
|---|---|
| instanceOf |
biological marker
ⓘ
fluorescent protein ⓘ protein ⓘ reporter gene ⓘ |
| discoveredInTaxon | Aequorea victoria ⓘ |
| emitsFluorescenceColor | green ⓘ |
| fluorescenceIs | autocatalytic ⓘ |
| formsStructure | beta-barrel ⓘ |
| hasAminoAcidLength | 238 amino acids ⓘ |
| hasApplicationIn |
cell biology
ⓘ
developmental biology ⓘ molecular biology ⓘ neuroscience ⓘ |
| hasChromophore | Ser65-Tyr66-Gly67 tripeptide ⓘ |
| hasCommonAbbreviation | GFP ⓘ |
| hasEmissionWavelength | 509 nm ⓘ |
| hasExcitationWavelength |
395 nm
ⓘ
475 nm ⓘ |
| hasMolecularWeight | about 27 kDa ⓘ |
| hasMutant |
EGFP
ⓘ
blue fluorescent protein ⓘ cyan fluorescent protein ⓘ enhanced GFP ⓘ
linked to:
green fluorescent protein
yellow fluorescent protein ⓘ |
| hasProperty |
forms stable beta-barrel scaffold
ⓘ
genetically encodable ⓘ intrinsically fluorescent ⓘ non-invasive reporter ⓘ |
| isEncodedBy | gfp gene ⓘ |
| isExcitedBy |
blue light
ⓘ
ultraviolet light ⓘ |
| isExpressedIn |
bacteria in recombinant form
ⓘ
mammalian cells in recombinant form ⓘ transgenic animals ⓘ yeast in recombinant form ⓘ |
| isUsedAs |
fusion tag
ⓘ
reporter protein ⓘ |
| isUsedFor |
biosensor construction
ⓘ
cell lineage tracing ⓘ live-cell imaging ⓘ promoter activity assays ⓘ protein localization studies ⓘ protein–protein interaction studies ⓘ visualizing gene expression ⓘ |
| isVisualizedBy | fluorescence microscopy ⓘ |
| NobelPrize2008LaureatesInclude |
Martin Chalfie
ⓘ
Osamu Shimomura ⓘ Roger Y. Tsien ⓘ |
| originallyIsolatedFrom | Aequorea victoria ⓘ |
| requires | molecular oxygen for chromophore maturation ⓘ |
| requiresCofactor | none external ⓘ |
| wasCoAwardedNobelPrizeIn |
Chemistry 2008
ⓘ
linked to:
Chemistry
|
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: green fluorescent protein Description of subject: Green fluorescent protein (GFP) is a naturally fluorescent protein originally found in jellyfish that is widely used as a marker in molecular and cell biology to visualize gene expression and protein localization.
Referenced by (3)
Full triples — surface form annotated when it differs from this entity's canonical label.