3DISCO tissue clearing method

E261427

solvent-based tissue clearing technique tissue clearing method

The 3DISCO tissue clearing method is a solvent-based technique that renders biological tissues transparent to enable high-resolution three-dimensional imaging of intact organs and organisms.

Try in SPARQL Jump to: Surface forms Disambiguation Statements Elicitation Referenced by

All labels observed (3)

Label	Occurrences
3DISCO tissue clearing method canonical	1
iDISCO tissue clearing method	1
uDISCO tissue clearing method	1

How this entity was disambiguated

This entity first appeared as the object of triple T2366518 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.

NED1 Entity disambiguation (via context triple) gpt-5-mini-2025-08-07

Target entity: 3DISCO tissue clearing method
Context triple: [CLARITY tissue-clearing method, relatedTo, 3DISCO tissue clearing method]

A. CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
B. Light-Space Modulator
Light-Space Modulator is an experimental kinetic sculpture by László Moholy-Nagy that explores movement, light, and shadow through rotating metal and glass forms.
C. Biomedical Optics Express
Biomedical Optics Express is a peer-reviewed scientific journal focusing on research and developments in the field of biomedical optics and photonics.
D. ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
E. The O2 complex
The O2 complex is a large entertainment district in London featuring the O2 Arena, cinemas, restaurants, bars, and various leisure and retail facilities housed under the iconic dome structure.
F. None of above. chosen
G. Unsure - the case is ambiguous/there is not enough information to decide.

NED2 Entity disambiguation (via description) gpt-5-mini-2025-08-07

Target entity: 3DISCO tissue clearing method
Target entity description: The 3DISCO tissue clearing method is a solvent-based technique that renders biological tissues transparent to enable high-resolution three-dimensional imaging of intact organs and organisms.

A. CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
B. Light-Space Modulator
Light-Space Modulator is an experimental kinetic sculpture by László Moholy-Nagy that explores movement, light, and shadow through rotating metal and glass forms.
C. Biomedical Optics Express
Biomedical Optics Express is a peer-reviewed scientific journal focusing on research and developments in the field of biomedical optics and photonics.
D. ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
E. The O2 complex
The O2 complex is a large entertainment district in London featuring the O2 Arena, cinemas, restaurants, bars, and various leisure and retail facilities housed under the iconic dome structure.
F. None of above. chosen

Statements (39)

Predicate	Object
instanceOf	solvent-based tissue clearing technique ⓘ tissue clearing method ⓘ
appliedTo	central nervous system tissues ⓘ embryos ⓘ mouse organs ⓘ
basedOn	lipid removal ⓘ organic solvent dehydration ⓘ refractive index matching ⓘ
compatibleWith	confocal microscopy ⓘ light-sheet fluorescence microscopy ⓘ
developedFor	whole-body imaging in small animals ⓘ whole-organ imaging ⓘ
developedIn	early 2010s ⓘ
effectOnTissue	increased optical transparency ⓘ volume reduction ⓘ
enables	high-resolution 3D imaging ⓘ
field	biological imaging ⓘ developmental biology ⓘ neuroanatomy ⓘ
hasAdvantage	high optical transparency ⓘ rapid clearing of large samples ⓘ
hasLimitation	quenching of endogenous fluorescence ⓘ tissue shrinkage ⓘ
optimizedFor	immunolabeled samples ⓘ
relatedTo	CLARITY tissue clearing method ⓘ linked to: CLARITY tissue-clearing method iDISCO tissue clearing method ⓘ linked to: 3DISCO tissue clearing method uDISCO tissue clearing method ⓘ linked to: 3DISCO tissue clearing method
requiresStep	graded dehydration in organic solvents ⓘ immersion in high refractive index medium ⓘ lipid extraction ⓘ
usedFor	rendering biological tissues transparent ⓘ three-dimensional imaging of intact organisms ⓘ three-dimensional imaging of intact organs ⓘ
usedIn	organ development studies ⓘ vascular network imaging ⓘ whole-brain mapping studies ⓘ
usesReagent	dibenzyl ether ⓘ dichloromethane ⓘ tetrahydrofuran ⓘ

How these facts were elicited

Referenced by (3)

Full triples — surface form annotated when it differs from this entity's canonical label.

CLARITY tissue-clearing method → relatedTo → 3DISCO tissue clearing method ⓘ

3DISCO tissue clearing method → relatedTo → uDISCO tissue clearing method ⓘ

linked to: 3DISCO tissue clearing method

3DISCO tissue clearing method → relatedTo → iDISCO tissue clearing method ⓘ