Statements (31)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
fluorescent stain
|
| gptkbp:alternativeName |
DAPI dye
DAPI stain |
| gptkbp:binds |
A-T rich regions of DNA
|
| gptkbp:chemicalFormula |
C16H15N5
|
| gptkbp:discoveredIn |
1970s
|
| gptkbp:emissionWavelength |
461 nm
|
| gptkbp:excitationWavelength |
358 nm
|
| gptkbp:fluorescence |
blue
|
| gptkbp:fullName |
4′,6-diamidino-2-phenylindole
|
| https://www.w3.org/2000/01/rdf-schema#label |
DAPI
|
| gptkbp:molecularWeight |
350.33 g/mol
|
| gptkbp:notableFor |
can pass through intact cell membranes
strong fluorescence enhancement upon DNA binding |
| gptkbp:riskFactor |
mutagenic
|
| gptkbp:solubility |
gptkb:water
|
| gptkbp:toxicity |
yes
|
| gptkbp:usedFor |
cell cycle analysis
DNA staining chromosome visualization detecting DNA in fixed cells identifying cell nuclei nuclear staining |
| gptkbp:usedIn |
cell imaging
fluorescence microscopy immunofluorescence flow cytometry apoptosis detection |
| gptkbp:bfsParent |
gptkb:Nuclei
gptkb:Dapodi_Metro_Station |
| gptkbp:bfsLayer |
7
|