polymerase chain reaction (PCR)
GPTKB entity
Statements (58)
| Predicate | Object |
|---|---|
| gptkbp:instance_of |
gptkb:Biology
|
| gptkbp:can_be_combined_with |
next-generation sequencing
Sanger sequencing gel electrophoresis |
| gptkbp:developed_by |
gptkb:Kary_Mullis
|
| gptkbp:first_introduced |
gptkb:1983
|
| gptkbp:has_applications_in |
DNA sequencing
cloning gene expression analysis pathogen detection |
| gptkbp:has_impact_on |
gptkb:forensic_science
medical diagnostics genetic research evolutionary biology |
| gptkbp:has_limitations |
contamination risk
requires specific primers not suitable for degraded DNA |
| https://www.w3.org/2000/01/rdf-schema#label |
polymerase chain reaction (PCR)
|
| gptkbp:involves |
gptkb:RNA_Polymerase
|
| gptkbp:involves_steps |
gptkb:cooperative
annealing denaturation |
| gptkbp:is_considered |
a revolutionary technique
|
| gptkbp:is_often_used_in |
environmental testing
food safety testing clinical diagnostics agricultural biotechnology |
| gptkbp:is_part_of |
gptkb:biotechnology
molecular diagnostics biomedical research genomics |
| gptkbp:is_subject_to |
patents
|
| gptkbp:is_taught_in |
biotechnology courses
genetics courses forensic science courses molecular biology courses |
| gptkbp:is_used_in |
clinical laboratories
research laboratories |
| gptkbp:modifications |
multiplex PCR
nested PCR quantitative PCR (q PCR) reverse transcription PCR (RT-PCR) |
| gptkbp:performed_in |
gptkb:Cooler
|
| gptkbp:requires |
nucleotides
DNA template primers |
| gptkbp:sensitivity |
temperature changes
p H levels |
| gptkbp:suitable_for |
RNA without reverse transcription
highly complex mixtures very large DNA fragments |
| gptkbp:used_for |
amplifying DNA
|
| gptkbp:used_in |
gptkb:forensic_science
gptkb:research genetic testing medical diagnostics |
| gptkbp:bfsParent |
gptkb:DNA
|
| gptkbp:bfsLayer |
3
|